Caco-2人结直肠腺癌细胞-杏鑫娱乐-【BTCC细胞库】杏鑫娱乐官网

Cell line name

Caco-2

Synonyms

CaCo-2; CACO-2; Caco 2; CACO 2; CACO2; CaCo2; CaCO2; Caco2; Caco-2/ATCC; Caco-II

Accession

BTCC-1033

Resource Identification Initiative

To cite this cell line use: Caco-2 (BTCC-1033)

Comments

Part of: AstraZeneca Colorectal cell line (AZCL) panel.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: ENCODE project common cell types; tier 3.
Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Virology: Highly susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=15316659).
Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257; PubMed=34339474).
Doubling time: 51.41 hours (PubMed=25944804); 3-4 days (ATCC=HTB-37); ~60-70 hours (CLS=300137); ~80 hours (DSMZ=ACC-169); ~32 hours (PBCF).
Microsatellite instability: Stable (MSS) (PubMed=24042735; PubMed=24755471; PubMed=25926053; PubMed=28683746).
Omics: CTCF ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Transcriptome analysis by serial analysis of gene expression (SAGE).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.

Sequence variations

Mutation; HGNC; 583; APC; Simple; p.Gln1367Ter (c.4099C>T); ClinVar=VCV000376060; Zygosity=Homozygous (PubMed=24755471; DepMap).
Mutation; HGNC; 2514; CTNNB1; Simple; p.Gly245Ala (c.734G>C); Zygosity=Heterozygous (PubMed=9294210).
Mutation; HGNC; 6770; SMAD4; Simple; p.Asp351His (c.1051G>C); ClinVar=VCV000375998; Zygosity=Homozygous (PubMed=24755471; DepMap).
Mutation; HGNC; 11998; TP53; Simple; p.Glu204Ter (c.610G>T); ClinVar=VCV000977783; Zygosity=Homozygous (PubMed=16418264; PubMed=24755471; PubMed=28683746; DepMap).

HLA typing

Source: PubMed=25960936

Class I
HLA-A	A*02:01,02:01
HLA-B	B*15:05,56/39
HLA-C	C*04:01,04:01

Source: PubMed=26589293

Class I
HLA-A	A*02:01,02:01
HLA-B	B*15:01,15:01
HLA-C	C*04:01,04:01

Source: CLS=300137

Class I
HLA-A	A*02:01:01
HLA-B	B*15:01:01
HLA-C	C*04:01:01
Class II
HLA-DP	DPB1*04:01:01
HLA-DQ	DQA1*03:01:01
	DQB1*03:02:01
HLA-DR	DRB1*04:04:01

Genome ancestry

Source: PubMed=30894373

Origin	% genome

African	3.33
Native American	0
East Asian, North	4.59
East Asian, South	0
South Asian	0
European, North	47.35
European, South	44.73

Disease

Colon adenocarcinoma (NCIt: C4349)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Children:


CVCL_B0YL (Abcam Caco-2 ACE2 KO)	CVCL_C0BL (Abcam Caco-2 BMP2 KO)	CVCL_C0BM (Abcam Caco-2 TMPRSS2 KO)
CVCL_1096 (C2BBe1)	CVCL_Z582 (C2BBe2)	CVCL_YP11 (Caco-2 Cu Pro-R)
CVCL_YP12 (Caco-2 CuSO4-R)	CVCL_C0R8 (Caco-2 DUSP6 KO)	CVCL_C8A0 (Caco-2 EJ6)
CVCL_HA82 (Caco-2-RCAr clone 2)	CVCL_B6E1 (Caco-2-SF)	CVCL_0232 (Caco-2/15)
CVCL_Z578 (Caco-2/3)	CVCL_Z579 (Caco-2/PD-10)	CVCL_Z580 (Caco-2/PD-7)
CVCL_Z581 (Caco-2/PF-11)	CVCL_0233 (Caco-2/TC-7)

Sex of cell

Male

Age at sampling

72Y

Category

Cancer cell line

STR profile

Source(s): AddexBio; ATCC; CCRID; CLS; DSMZ; ECACC; PubMed=11416159; PubMed=25877200; PubMed=25926053; RCB

Markers:

Amelogenin	X
CSF1PO	11
D1S1656	15,16
D2S1338	17,25
D3S1358	14,17
D5S818	12,13
D7S820	11,12 (ATCC; CCRID; CLS; DSMZ; ECACC; PubMed=25877200; PubMed=25926053; RCB) 12 (AddexBio)
D8S1179	12 (DSMZ; PubMed=11416159) 12,14 (CCRID; CLS; PubMed=25877200)
D10S1248	13,15
D12S391	17,23
D13S317	11,13,14
D16S539	12,13
D18S51	12
D19S433	15
D21S11	30 (DSMZ; PubMed=11416159; PubMed=25877200) 30,32 (CCRID; CLS; PubMed=25926053)
D22S1045	15,16
FGA	19
Penta D	9 (DSMZ) 9,11 (PubMed=25877200)
Penta E	7
SE33	21
TH01	6
TPOX	9,11
vWA	16,18

Web pages

https://en.wikipedia.org/wiki/Caco-2
https://www.atcc.org/en/support/technical-support/faqs/growth-rate-of-atcc-htb-37
https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-htb-37
https://www.phe-culturecollections.org.uk/media/171256/caco-2-cell-line-profile.pdf
https://www.proteinatlas.org/learn/cellines
http://www.sygnis.com/caco-2/
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-37.html
http://www.cyprotex.com/admepk/in-vitro-permeability/caco-2-permeability/
https://www.synapse.org/#!Synapse:syn31544480
https://tcpaportal.org/mclp/

Publications

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3349466
Chantret I., Barbat A., Dussaulx E., Brattain M.G., Zweibaum A.
Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.
Cancer Res. 48:1936-1942(1988)

PubMed=2914637; DOI=10.1016/0016-5085(89)90897-4
Hidalgo I.J., Raub T.J., Borchardt R.T.
Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability.
Gastroenterology 96:736-749(1989)

PubMed=7764660; DOI=10.1007/BF00749813
Hashimoto K., Shimizu M.
Epithelial properties of human intestinal Caco-2 cells cultured in a serum-free medium.
Cytotechnology 13:175-184(1993)

PubMed=8508948; DOI=10.1111/j.1432-0436.1993.tb00645.x
de Bruine A.P., de Vries J.E., Dinjens W.N.M., Moerkerk P.T.M., van der Linden E.P.M., Pijls M.M.J., ten Kate J., Bosman F.T.
Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine.
Differentiation 53:51-60(1993)

PubMed=9294210; DOI=10.1073/pnas.94.19.10330
Ilyas M., Tomlinson I.P.M., Rowan A.J., Pignatelli M., Bodmer W.F.
Beta-catenin mutations in cell lines established from human colorectal cancers.
Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997)

PubMed=10612807; DOI=10.1002/(SICI)1098-2264(200002)27:2<183::aid-gcc10>3.0.CO;2-P
Ghadimi B.M., Sackett D.L., Difilippantonio M.J., Schrock E., Neumann T., Jauho A., Auer G., Ried T.
Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.
Genes Chromosomes Cancer 27:183-190(2000)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=12584437; DOI=10.1159/000068544
Melcher R., Koehler S., Steinlein C., Schmid M., Mueller C.R., Luehrs H., Menzel T., Scheppach W., Moerk H., Scheurlen M., Koehrle J., Al-Taie O.
Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway.
Cytogenet. Genome Res. 98:22-28(2002)

PubMed=14599474; DOI=10.1016/S0887-2333(03)00095-X
Ranaldi G., Consalvo R., Sambuy Y., Scarino M.L.
Permeability characteristics of parental and clonal human intestinal Caco-2 cell lines differentiated in serum-supplemented and serum-free media.
Toxicol. In Vitro 17:761-767(2003)

PubMed=15316659; DOI=10.1007/s00018-004-4222-9
Cinatl J. Jr., Hoever G., Morgenstern B., Preiser W., Vogel J.-U., Hofmann W.-K., Bauer G., Michaelis M., Rabenau H.F., Doerr H.-W.
Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus.
Cell. Mol. Life Sci. 61:2100-2112(2004)

Cell line collections (Providers)	Abcam; ab275464 - Discontinued AddexBio; C0009009/29 ATCC; HTB-37 BCRJ; 0059 CCTCC; GDC0153 CLS; 300137 DSMZ; ACC-169 ECACC; 09042001 ECACC; 86010202 IBRC; C10094 ICLC; HTL97023 IZSLER; BS TCL 87 KCB; KCB 200710YJ KCLB; 30037.1 NCBI_Iran; C139 RCB; RCB0988
Chemistry resources	ChEMBL-Cells; CHEMBL3307519 ChEMBL-Targets; CHEMBL614058 PharmacoDB; Caco2_161_2019 PubChem_Cell_line; CVCL_0025
Encyclopedic resources	Wikidata; Q5016050
Experimental variables resources	EFO; EFO_0001099

质检报告（COA）
Markers:

Amelogenin	X
CSF1PO	11
D1S1656	15,16
D2S1338	17,25
D3S1358	14,17
D5S818	12,13
D7S820	11,12 (ATCC; CCRID; CLS; DSMZ; ECACC; PubMed=25877200; PubMed=25926053; RCB) 12 (AddexBio)
D8S1179	12 (DSMZ; PubMed=11416159) 12,14 (CCRID; CLS; PubMed=25877200)
D10S1248	13,15
D12S391	17,23
D13S317	11,13,14
D16S539	12,13
D18S51	12
D19S433	15
D21S11	30 (DSMZ; PubMed=11416159; PubMed=25877200) 30,32 (CCRID; CLS; PubMed=25926053)
D22S1045	15,16
FGA	19
Penta D	9 (DSMZ) 9,11 (PubMed=25877200)
Penta E	7
SE33	21
TH01	6
TPOX	9,11
vWA	16,18

Caco-2人结直肠腺癌细胞(STR鉴定正确)

详细资料说明书下载质检报告（COA）

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