• PA-1人卵巢畸胎瘤细胞(STR鉴定正确)

    价 格:¥1800

    期 货:现货(冻存管发2支)

    保藏中心🛀🏻:BTCC

    资源编号:BTCC-1336

    产地:中国

    套 餐⚛️:

    T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

    详细资料说明书下载质检报告(COA)

    Cell line namePA-1
    SynonymsPA1; PA I; PAI
    AccessionBTCC-1336
    Resource Identification InitiativeTo cite this cell line use: PA-1 (BTCC-1336)
    CommentsPart of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
    Part of: COSMIC cell lines project.
    Part of: KuDOS 95 cell line panel.
    Doubling time: 36 hours (PubMed=2653399); ~24 hours (Note=Lot 01212002) (JCRB=JCRB9061); 22.77 hours (GrayJW panel).
    Microsatellite instability: Stable (MSS) (Sanger).
    Omics: Deep exome analysis.
    Omics: Deep quantitative proteome analysis.
    Omics: DNA methylation analysis.
    Omics: SNP array analysis.
    Omics: Transcriptome analysis by microarray.
    Omics: Transcriptome analysis by RNAseq.
    Caution: TP53 mutation indicated to be at p.Asn239Asp (c.715A>G) in DOI=10.11418/jtca1981.16.3_173 and PubMed=9359923.
    Discontinued: TKG; TKG 0573; true.
    Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
    Sequence variations
    • Mutation; HGNC; 7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous; Note=Not found in early passages (PubMed=6740333).
    • Mutation; HGNC; 11998; TP53; Simple; p.Pro316Pro (c.948C>T); ClinVar=VCV000750727; Zygosity=Heterozygous (Cosmic-CLP).
    HLA typingSource: PubMed=26589293
    Class I
    HLA-AA*68:02,68:02
    HLA-BB*37:04,44:03
    HLA-CC*07:01,07:01
    Class II
    HLA-DQDQB1*04:01,04:01
    HLA-DRDRB1*03:02,03:02
    Genome ancestrySource: PubMed=30894373

    Origin% genome


    African21.08
    Native American17.69
    East Asian, North1.54
    East Asian, South0.7
    South Asian0.4
    European, North26.01
    European, South32.58
    DiseaseOvarian mixed germ cell tumor (NCIt: C8114)
    Mixed germ cell tumor (ORDO: Orphanet_180234)
    Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
    HierarchyChildren:



    CVCL_1T15 (222)CVCL_4992 (CH1 [Human ovarian carcinoma])CVCL_3121 (PA-1/6TG-r)
    Sex of cellFemale
    Age at sampling12Y
    CategoryCancer cell line
    STR profileSource(s): ATCC; CCRID; CLS; COG; Cosmic-CLP; DepMap; ECACC; JCRB; PubMed=25877200

    Markers:
    AmelogeninX
    CSF1PO9,12
    D2S133824
    D3S135815
    D5S81811
    D7S8209
    D8S117914,15
    D13S3179,10 (ATCC; CCRID; CLS; COG; Cosmic-CLP; DepMap; ECACC; PubMed=25877200)
    9,10,13 (JCRB)
    D16S5399 (CLS)
    9,12 (ATCC; CCRID; COG; Cosmic-CLP; DepMap; ECACC; JCRB; PubMed=25877200)
    D18S5115,18
    D19S43313
    D21S1129,31.2
    FGA24
    Penta D9,12
    Penta E14,20
    TH017,9
    TPOX11
    vWA15,17

    Run an STR similarity search on this cell line
    Publications

    PubMed=6931103; DOI=10.1002/ijc.2910250104
    Zeuthen J., Norgaard J.O.R., Avner P., Fellous M., Wartiovaara J., Vaheri A., Rosen A., Giovanella B.C.
    Characterization of a human ovarian teratocarcinoma-derived cell line.
    Int. J. Cancer 25:19-32(1980)

    PubMed=6740333; DOI=10.1126/science.6740333
    Tainsky M.A., Cooper C.S., Giovanella B.C., Vande Woude G.F.
    An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells.
    Science 225:643-645(1984)

    PubMed=2653399; DOI=10.1038/bjc.1989.108
    Hills C.A., Kelland L.R., Abel G., Siracky J., Wilson A.P., Harrap K.R.
    Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes.
    Br. J. Cancer 59:527-534(1989)

    DOI=10.11418/jtca1981.16.3_173
    Mihara K., Miyazaki M., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ohashi R., Namba M.
    The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
    Tissue Cult. Res. Commun. 16:173-178(1997)

    PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::aid-mc5>3.0.CO;2-D
    Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
    Screening the p53 status of human cell lines using a yeast functional assay.
    Mol. Carcinog. 19:243-253(1997)

    PubMed=9359923; DOI=10.18926/AMO/30789
    Mihara K., Miyazaki M., Kondo T., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ishioka C., Namba M.
    Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
    Acta Med. Okayama 51:261-265(1997)

    PubMed=9698466; DOI=10.1006/gyno.1998.5039
    Maxwell G.L., Risinger J.I., Tong B., Shaw H., Barrett J.C., Berchuck A., Futreal P.A.
    Mutation of the PTEN tumor suppressor gene is not a feature of ovarian cancers.
    Gynecol. Oncol. 70:13-16(1998)

    PubMed=10567668; DOI=10.3892/ijmm.4.6.597
    Gao C., Miyazaki M., Li J.W., Tsuji T., Inoue Y., Namba M.
    Cytogenetic characteristics and p53 gene status of human teratocarcinoma PA-1 cells in 407-445 passages.
    Int. J. Mol. Med. 4:597-600(1999)

    PubMed=11314036; DOI=10.1038/sj.onc.1204211
    Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.
    Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
    Oncogene 20:1005-1009(2001)

    PubMed=11330945; DOI=10.1006/gyno.2001.6132
    Watanabe T., Imoto I., Kosugi Y., Ishiwata I., Inoue S., Takayama M., Sato A., Inazawa J.
    A novel amplification at 17q21-23 in ovarian cancer cell lines detected by comparative genomic hybridization.
    Gynecol. Oncol. 81:172-177(2001)

    PubMed=15677628; DOI=10.1093/carcin/bgi032
    Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
    Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
    Carcinogenesis 26:923-930(2005)

    PubMed=16080959; DOI=10.1016/j.cancergencyto.2005.01.003
    Sarraf S., Tejada R., Abawi M., Oberst M., Dennis T., Simon K.C., Blancato J.
    The human ovarian teratocarcinoma cell line PA-1 demonstrates a single translocation: analysis with fluorescence in situ hybridization, spectral karyotyping, and bacterial artificial chromosome microarray.
    Cancer Genet. Cytogenet. 161:63-69(2005)

    PubMed=20164919; DOI=10.1038/nature08768
    Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
    Signatures of mutation and selection in the cancer genome.
    Nature 463:893-898(2010)

    PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
    Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
    DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
    Gynecol. Oncol. 127:241-248(2012)

    PubMed=23415752; DOI=10.1016/j.molonc.2012.12.007
    Stordal B., Timms K., Farrelly A., Gallagher D., Busschots S., Renaud M., Thery J., Williams D., Potter J., Tran T., Korpanty G., Cremona M., Carey M.S., Li J., Li Y., Aslan O., O'Leary J.J., Mills G.B., Hennessy B.T.
    BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation.
    Mol. Oncol. 7:567-579(2013)

    PubMed=25485619; DOI=10.1038/nbt.3080
    Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
    A comprehensive transcriptional portrait of human cancer cell lines.
    Nat. Biotechnol. 33:306-312(2015)

    PubMed=26589293; DOI=10.1186/s13073-015-0240-5
    Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
    TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
    Genome Med. 7:118.1-118.7(2015)

    PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
    Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
    A landscape of pharmacogenomic interactions in cancer.
    Cell 166:740-754(2016)

    PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
    Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
    An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
    Cancer Res. 79:1263-1273(2019)

    PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
    Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
    Pan-cancer proteomic map of 949 human cell lines.
    Cancer Cell 40:835-849.e8(2022)

    Cross-references
    Cell line databases/resourcesCLO; CLO_0008350
    MCCL; MCC:0000377
    CLDB; cl3856
    CLDB; cl3857
    CLDB; cl3858
    CLDB; cl3859
    CCRID; 1101HUM-PUMC000021
    Cell_Model_Passport; SIDM01140
    CGH-DB; 9358-4
    Cosmic-CLP; 909255
    DepMap; ACH-001374
    LINCS_LDP; LCL-1416
    Anatomy/cell type resourcesBTO; BTO:0001591
    Biological sample resourcesBioSample; SAMN01821586
    BioSample; SAMN03473209
    BioSample; SAMN03472415
    Cell line collections (Providers)ATCC; CRL-1572
    BCRC; 60063
    CLS; 300402
    ECACC; 90013101
    ICLC; HTL97002
    IZSLER; BS TCL 179
    JCRB; JCRB9061
    RCB; RCB1946 - Discontinued
    TKG; TKG 0573
    Chemistry resourcesChEMBL-Cells; CHEMBL3308812
    ChEMBL-Targets; CHEMBL614949
    GDSC; 909255
    PharmacoDB; PA1_1235_2019
    PubChem_Cell_line; CVCL_0479
    Encyclopedic resourcesWikidata; Q54937331
    Experimental variables resourcesEFO; EFO_0006465

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